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Structured Review

Proteintech muc 2
Muc 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muc+2/MUC2+Antibody/pm41747917-88-24-26
Average 96 stars, based on 256 article reviews
muc 2 - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Incubation:

Article Title: Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice.
Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). .. Membranes were incubated overnight at 4 °C with primary antibodies against ZO-1 (1:1000, Cat# 21773-1-AP, Proteintech), MUC-2 (1:1000, Cat# 27675-1-AP, Proteintech), Occludin (1:1000, Cat# 27260-1-AP, Proteintech), cGAS (1:1000, Cat# 26416-1- AP, Proteintech), STING (1:1000, Cat# 19851-1-AP, Proteintech), C1q (1:1000, Cat# 11602-1-AP, Proteintech), PSD95 (1:1000, Cat# ab18258, Abcam), SYN (1:1000, Cat# 17785-1-AP, Proteintech), ACOD1 (1:1000, Cat#17805, CST), RNF5 (1:1000, Cat#A8351, Abclonal), Ubiquitin (UB) (1:1000, Cat# 10201-2-AP, Proteintech), β-actin (1:1000, Cat# 66009-1- Ig Proteintech),GAPDH (1:1000, Cat# 60004-1-Ig, Proteintech) and a custom-made antibody for the K150 ubiquitination site on STING (1:1000, Cat#RPM0002, Abclonal). ..

Article Title: Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice
Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). .. Membranes were incubated overnight at 4 °C with primary antibodies against ZO-1 (1:1000, Cat# 21773-1-AP, Proteintech), MUC-2 (1:1000, Cat# 27675-1-AP, Proteintech), Occludin (1:1000, Cat# 27260-1-AP, Proteintech), cGAS (1:1000, Cat# 26416-1-AP, Proteintech), STING (1:1000, Cat# 19851-1-AP, Proteintech), C1q (1:1000, Cat# 11602-1-AP, Proteintech), PSD95 (1:1000, Cat# ab18258, Abcam), SYN (1:1000, Cat# 17785-1-AP, Proteintech), ACOD1 (1:1000, Cat#17805, CST), RNF5 (1:1000, Cat#A8351, Abclonal), Ubiquitin (UB) (1:1000, Cat# 10201-2-AP, Proteintech), β-actin (1:1000, Cat# 66009-1-Ig Proteintech),GAPDH (1:1000, Cat# 60004-1-Ig, Proteintech) and a custom-made antibody for the K150 ubiquitination site on STING (1:1000, Cat# RPM0002, Abclonal). ..

Article Title: Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model.
Article Snippet: .. Sections were subsequently blocked with 5% goat serum and incubated overnight at 4 °C with the following primary antibodies diluted at 1:100: anti-TNF-α (ab215188, Abcam, USA), anti-IL-17 (13838 S, Cell Signaling Technology, USA), anti-IL-22 (A6212, ABclonal, China), anti-GM-CSF (505504, BioLegend, USA), and MUC-2 (27675-1-AP, Proteintech, China). ..

Article Title: Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model
Article Snippet: .. Sections were subsequently blocked with 5% goat serum and incubated overnight at 4 °C with the following primary antibodies diluted at 1:100: anti-TNF-α (ab215188, Abcam, USA), anti-IL-17 (13838 S, Cell Signaling Technology, USA), anti-IL-22 (A6212, ABclonal, China), anti-GM-CSF (505504, BioLegend, USA), and MUC-2 (27675-1-AP, Proteintech, China). ..

Article Title: Resveratrol attenuates Citrobacter rodentium -infected colitis by remodeling the gut microbiota structure and inhibiting CD4 + cell activation in mice.
Article Snippet: Infectious enteritis is a chronic inflammatory condition of the intestine, mainly characterized by immune cell infiltration and gut microbiota dysbiosis.. Resveratrol, a natural polyphenol compound from grapes, berries and peanuts, is known for its antioxidant and anti-inflammatory properties.. This study investigated the effects of resveratrol in a murine model of Citrobacter rodentium-induced colitis.

Article Title: Probiotic-derived extracellular vesicles attenuate cholestatic liver damage via gut-liver axis.
Article Snippet: .. For immunofluorescence staining, tissues were fixed with 4% paraformaldehyde and incubated with antibodies against FXR (sc-25309, Santa Cruz, USA), F4/80 (ab300421, Abcam, USA) and Muc-2 (27675–1-AP, Proteintech, USA). ..

Ubiquitin Proteomics:

Article Title: Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice.
Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). .. Membranes were incubated overnight at 4 °C with primary antibodies against ZO-1 (1:1000, Cat# 21773-1-AP, Proteintech), MUC-2 (1:1000, Cat# 27675-1-AP, Proteintech), Occludin (1:1000, Cat# 27260-1-AP, Proteintech), cGAS (1:1000, Cat# 26416-1- AP, Proteintech), STING (1:1000, Cat# 19851-1-AP, Proteintech), C1q (1:1000, Cat# 11602-1-AP, Proteintech), PSD95 (1:1000, Cat# ab18258, Abcam), SYN (1:1000, Cat# 17785-1-AP, Proteintech), ACOD1 (1:1000, Cat#17805, CST), RNF5 (1:1000, Cat#A8351, Abclonal), Ubiquitin (UB) (1:1000, Cat# 10201-2-AP, Proteintech), β-actin (1:1000, Cat# 66009-1- Ig Proteintech),GAPDH (1:1000, Cat# 60004-1-Ig, Proteintech) and a custom-made antibody for the K150 ubiquitination site on STING (1:1000, Cat#RPM0002, Abclonal). ..

Article Title: Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice
Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). .. Membranes were incubated overnight at 4 °C with primary antibodies against ZO-1 (1:1000, Cat# 21773-1-AP, Proteintech), MUC-2 (1:1000, Cat# 27675-1-AP, Proteintech), Occludin (1:1000, Cat# 27260-1-AP, Proteintech), cGAS (1:1000, Cat# 26416-1-AP, Proteintech), STING (1:1000, Cat# 19851-1-AP, Proteintech), C1q (1:1000, Cat# 11602-1-AP, Proteintech), PSD95 (1:1000, Cat# ab18258, Abcam), SYN (1:1000, Cat# 17785-1-AP, Proteintech), ACOD1 (1:1000, Cat#17805, CST), RNF5 (1:1000, Cat#A8351, Abclonal), Ubiquitin (UB) (1:1000, Cat# 10201-2-AP, Proteintech), β-actin (1:1000, Cat# 66009-1-Ig Proteintech),GAPDH (1:1000, Cat# 60004-1-Ig, Proteintech) and a custom-made antibody for the K150 ubiquitination site on STING (1:1000, Cat# RPM0002, Abclonal). ..

other:

Article Title: Yuzhi Zhixue Granules regulate the JAK/STAT pathway to affect T-cell differentiation in the colon of ulcerative colitis.
Article Snippet: Occludin (66,378), Claudin-1 (68,969), ZO-1, Muc-2 (27,675–1-AP), STAT3 (88 kDa, 60,199–1-Ig), STAT6 (95 kDa, 66,717–1-Ig), and murine monoclonal antibody to GAPDH (36 kDa, 60,004–1-Ig) were obtained from Proteintech, USA.

Blocking Assay:

Article Title: Resveratrol attenuates Citrobacter rodentium -infected colitis by remodeling the gut microbiota structure and inhibiting CD4 + cell activation in mice.
Article Snippet: Infectious enteritis is a chronic inflammatory condition of the intestine, mainly characterized by immune cell infiltration and gut microbiota dysbiosis.. Resveratrol, a natural polyphenol compound from grapes, berries and peanuts, is known for its antioxidant and anti-inflammatory properties.. This study investigated the effects of resveratrol in a murine model of Citrobacter rodentium-induced colitis.

Immunofluorescence:

Article Title: Probiotic-derived extracellular vesicles attenuate cholestatic liver damage via gut-liver axis.
Article Snippet: .. For immunofluorescence staining, tissues were fixed with 4% paraformaldehyde and incubated with antibodies against FXR (sc-25309, Santa Cruz, USA), F4/80 (ab300421, Abcam, USA) and Muc-2 (27675–1-AP, Proteintech, USA). ..

Staining:

Article Title: Probiotic-derived extracellular vesicles attenuate cholestatic liver damage via gut-liver axis.
Article Snippet: .. For immunofluorescence staining, tissues were fixed with 4% paraformaldehyde and incubated with antibodies against FXR (sc-25309, Santa Cruz, USA), F4/80 (ab300421, Abcam, USA) and Muc-2 (27675–1-AP, Proteintech, USA). ..



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Image Search Results


Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the mucin MUC-2 (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Oral Microbiology

Article Title: Aggregatibacter actinomycetemcomitans exacerbates colitis and perturbs the gut microbiota in a murine model​

doi: 10.1080/20002297.2026.2613536

Figure Lengend Snippet: Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the mucin MUC-2 (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The ZO-1 and MUC-2 protein-targeting rabbit antibodies (Servicebio, China) were applied to the 4 μm-thick tissue sections, which were subsequently incubated overnight at 4 °C, washed with phosphate-buffered saline, and then treated with anti-rabbit fluorescent secondary antibodies (Servicebio, China) at 37 °C for 1 h. Following washing, the tissue nuclei were re-stained with 4-methyl-6-methyl-2-phenylindole (DAPI).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Software